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Image Search Results
Journal: Oncotarget
Article Title: Integrin signaling via FAK-Src controls cytokinetic abscission by decelerating PLK1 degradation and subsequent recruitment of CEP55 at the midbody
doi: 10.18632/oncotarget.9003
Figure Lengend Snippet: ( A , B , F ) Representative immunofluorescence micrographs illustrating the distribution of selected proteins in the F/M region (labeled with Aurora B) at the beginning of cytokinesis and after 60 minutes incubation of BJ cells adhering to fibronectin or kept in suspension. (A) MKLP1 (green, upper left panel) and CEP55 (green, upper right panel), (B) TSG101 (green, lower left panel) and ALIX (red, lower right panel), (F) CHMP4B (green). The square frames show the midbody area at higher magnification. Arrows indicate midbody regions in the merged pictures. Nuclei were stained with DAPI (blue). Mean % ± SD of the number of mitotic cells in cytokinesis lacking ( C ) MKLP1 and CEP55, ( D ) TSG101 and ALIX and ( G ) CHMP4B in the F/M regions. ***, **, and * represent P -value less than 0.001, 0.01 and 0.05, respectively. ( E ) Representative western blot of cell lysates illustrating the level of ALIX and TSG101 in exponentially proliferating cells (Control) and synchronized mitotic cells directly after isolation (Zero) and following two hours incubation under the adhesion or suspension conditions.
Article Snippet: The blots were probed with primary antibodies to Aurora B (1:1000, ab-2254, Abcam), CEP55 (1:1000, sc-134622, Santa Cruz), PLK1 (1:1000, GTX 104302, GENTEX), ALIX (1:500, sc-53538, Santa Cruz),
Techniques: Immunofluorescence, Labeling, Incubation, Suspension, Staining, Western Blot, Control, Isolation